An integrated lab-on-chip platform was developed to detect ovarian clear cell carcinoma (OCCC) biomarker FXYD2. This single chip automatically deals with ascites, captures cancer cells, isolates messenger RNA, and completes quantitative reverse-transcription polymerase chain reaction.
A benchtop protocol of this system could have a limit of quantification as low as 100 copies per sample. A simple cycle threshold ratio index can distinguish high FXYD2 expressions cells by normalizing to a housekeeping gene, GAPDH.
