E.Coli detection using lateral flow strips

Source: Lateral flow device for water fecal pollution assessment: from troubleshooting of its microfluidics using bioluminescence to colorimetric monitoring of generic Escherichia coli

Researchers have developed a potentially life saving technique to test for all variants of Escherichia coli in water to detect fecal pollution. They achieved this modifying standard Lateral Flow Strips to use Gold Nanoparticles with polyclonal antibodies. It has an easily interpretable colorimetric output for detection of the E. Coli. They also developed a bioluminescent characterization method to analyze bacterial flow through different lateral flow materials. Adding a simple filtration system increases the system’s LOD and sensitivity. This low cost test produces results within 15 minutes.

Read the paper below to read more about the fabrication of the strip.

Source: Lateral flow device for water fecal pollution assessment: from troubleshooting of its microfluidics using bioluminescence to colorimetric monitoring of generic Escherichia coli